Part:BBa_K1855000

Lpp-OmpA Zif268

A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (Zif268 1AAY fragment),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to CCGCCCNCGC on the cell surface of E.coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 558
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 519
  
• 1000
  COMPATIBLE WITH RFC[1000]

Zif268

Cells transformed with the Lpp-OmpA-Zif268 construct; wild-type, uninduced and induced in order from left to right. The cells were washed first with an Anti-FLAG antibody, followed by an Anti-mouse fluorescent antibody. The cells were then observed under a fluorescence microscoopy. The uninduced cells show the expected result, as did the induced; however the wild type appears to show high fluorescence. The striped on the induced cells are likely due to poor slide preparation, and the result from the wild-type is likely due to poor wash to remove the antibody.